User:Madeline Hartman/Notebook/Dr. Hartings Lab/2014/02/06: Difference between revisions
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==Entry title== | ==Entry title== | ||
Ran a trial in OceanView for Absorbance readings. | |||
Run at 25°C set through Qpod | |||
#Run A Wizard | |||
#Select spectroscopy | |||
#JAZ is the correct hardware device. Click next | |||
#Select absorbance and click next | |||
#Select integration time as 1 ms | |||
#Select scans to average 10 | |||
#Click next | |||
#Open lamp. Click lightbulb to store reference spectrum. Click next | |||
#Close lamp. Click the lightbulb to store dark spectrum. Click finish | |||
#Open lamp. Click save graph to files. Configure | |||
#Save the first available scan every 1 minute | |||
#Stop after 2 hours | |||
#Save to directory in a dropbox folder - 02-06- MeOH-25 | |||
#File format- Tab separated with headers | |||
#Create file name - mb_opd_phosphate_meoh_h202_25 | |||
#Click apply | |||
# When ready to run, click play button, then click the save graph to files button a second time. | |||
Cuvette at start time contains- | |||
# 0.675 mL (3.4 mg OPD in 3.4 mL MeOH) | |||
# 0.75 mL dilute H2O2 (0.1135 μL H2O2 and 0.8865 μL H2O) | |||
First reading taken after 45 seconds. | |||
0.225 mL of Mb added after 2 minutes (between readings 2 and 3) (1 mg Mb/KCl/Phosphate buffer in 1 mL MeOH) | |||
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__NOTOC__ | __NOTOC__ |
Revision as of 08:56, 6 February 2014
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Entry titleRan a trial in OceanView for Absorbance readings. Run at 25°C set through Qpod
Cuvette at start time contains-
First reading taken after 45 seconds. 0.225 mL of Mb added after 2 minutes (between readings 2 and 3) (1 mg Mb/KCl/Phosphate buffer in 1 mL MeOH)
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