User:Madeline Hartman/Notebook/Dr. Hartings Lab/2013/01/29: Difference between revisions
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==Entry title== | ==Entry title== | ||
Gel electrophoresis was run to test PCR | |||
1% Agarose Gel- .25 g agarose | |||
25 mL electrophorsis buffer | |||
Microwave for 45 seconds to dissolve | |||
Lane 2- 6 μL marker | |||
Lane 3- 1 μL dye + 5 μL original DNA | |||
Lane 4- 1 μL dye + 5μL forward/reverse DNA | |||
Run at 100 V for 60 minutes | |||
Results: | |||
Original- No Band | |||
Forward/Reverse- Band | |||
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__NOTOC__ | __NOTOC__ | ||
Revision as of 12:13, 22 September 2013
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Entry titleGel electrophoresis was run to test PCR 1% Agarose Gel- .25 g agarose 25 mL electrophorsis buffer Microwave for 45 seconds to dissolve Lane 2- 6 μL marker Lane 3- 1 μL dye + 5 μL original DNA Lane 4- 1 μL dye + 5μL forward/reverse DNA Run at 100 V for 60 minutes Results: Original- No Band Forward/Reverse- Band
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