User:Madeline Hartman/Notebook/Dr. Hartings Lab/2012/11/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Entry title== | ==Entry title== | ||
Cell Transformation was performed | |||
# BL21(DE3) cells thawed gently on ice | |||
# The competent cells were mixed. 100 μL cells were added to three separate 14-ml BD Falcon polypropylene round bottom tubes. | |||
# The three tubes were incubated on ice for 10 minutes, swirling gently ever 2 minutes | |||
# 5 μL, 10 μL, and 15 μL of ligated DNA were added to one of the three tubes, and swirled gently | |||
# The three tubes were incubated on ice for 30 minutes | |||
# Each of the three tubes was heat-pulsed for 45 seconds in a 42°C water bath | |||
# The three tubes were incubated on ice for 2 minutes | |||
# 0.9 ml of LB medium was added to each of the three tubes and the tubes were incubated at 37 °C for 1 hour while shaking at 225 rpm. | |||
# 200 μL of cells were plated and spread with a sterile spreader | |||
# The cells were incubated overnight at 37°C | |||
Cell transformation was unsuccessful | |||
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Latest revision as of 22:19, 26 September 2017
Project name | Main project page Previous entry Next entry |
Entry titleCell Transformation was performed
Cell transformation was unsuccessful |