User:Madeline Hartman/Notebook/Dr. Hartings Lab/2012/11/13: Difference between revisions
From OpenWetWare
(Autocreate 2012/11/13 Entry for User:Madeline_Hartman/Notebook/Dr._Hartings_Lab) |
|||
| Line 7: | Line 7: | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==Entry title== | ==Entry title== | ||
Gel electrophoresis was run on Tube 1 (original) and Tube 2+3 (forward/reverse). | |||
Forward/reverse was successful, original was now. | |||
Gel | |||
Lane 1- Marker | |||
Lane 2- Original | |||
Lane 3- Forward/reverse | |||
Marker- 30 μL marker + 6 μL dye --> 6 μL added | |||
Original 5 μL DNA + 1μL dye --> 6 μL added | |||
Forward/reverse- 5μL DNA + 1μL dye --> 6 μL added | |||
# Gel was run for 45 minutes at 80 V with TAE buffer | |||
# Gel was removed from buffer and placed in ethidium bromide solution for 30 minutes while rocking | |||
# Gel was placed in rinse buffer for 15 minutes while rocking | |||
# Gel was viewed under UV light | |||
Revision as of 10:33, 4 December 2012
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Entry titleGel electrophoresis was run on Tube 1 (original) and Tube 2+3 (forward/reverse). Forward/reverse was successful, original was now. Gel Lane 1- Marker Lane 2- Original Lane 3- Forward/reverse Marker- 30 μL marker + 6 μL dye --> 6 μL added Original 5 μL DNA + 1μL dye --> 6 μL added Forward/reverse- 5μL DNA + 1μL dye --> 6 μL added
| |
