User:Madeline Hartman/Notebook/Dr. Hartings Lab/2012/11/13
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==Entry title== | ==Entry title== | ||
| - | + | Gel electrophoresis was run on Tube 1 (original) and Tube 2+3 (forward/reverse). | |
| + | Forward/reverse was successful, original was now. | ||
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| + | Gel | ||
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| + | Lane 1- Marker | ||
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| + | Lane 2- Original | ||
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| + | Lane 3- Forward/reverse | ||
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| + | Marker- 30 μL marker + 6 μL dye --> 6 μL added | ||
| + | Original 5 μL DNA + 1μL dye --> 6 μL added | ||
| + | Forward/reverse- 5μL DNA + 1μL dye --> 6 μL added | ||
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| + | # Gel was run for 45 minutes at 80 V with TAE buffer | ||
| + | # Gel was removed from buffer and placed in ethidium bromide solution for 30 minutes while rocking | ||
| + | # Gel was placed in rinse buffer for 15 minutes while rocking | ||
| + | # Gel was viewed under UV light | ||
Revision as of 13:33, 4 December 2012
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Entry titleGel electrophoresis was run on Tube 1 (original) and Tube 2+3 (forward/reverse). Forward/reverse was successful, original was now. Gel Lane 1- Marker Lane 2- Original Lane 3- Forward/reverse Marker- 30 μL marker + 6 μL dye --> 6 μL added Original 5 μL DNA + 1μL dye --> 6 μL added Forward/reverse- 5μL DNA + 1μL dye --> 6 μL added
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