User:Madeline Hartman/Notebook/Dr. Hartings Lab/2012/11/06

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2012/11/06 Entry for User:Madeline_Hartman/Notebook/Dr._Hartings_Lab)
(Entry title)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Entry title==
-
* Insert content here...
+
I ran two PCR reactions with the following content:
 +
 
 +
# 40.6 μL distilled H2O
 +
# 5.0 μL 10x cloned Pfu buffer
 +
# 0.4 μL dNTPs (25 mM each)
 +
# 1.0 μL DNA template (100 ng/μL)
 +
# 1.0 μL Primer 1 (ng/μL)
 +
# 1.0 μL Primer 2 (ng/μL)
 +
# 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)

Revision as of 11:46, 4 December 2012

Project name Main project page
Next entry

Entry title

I ran two PCR reactions with the following content:

  1. 40.6 μL distilled H2O
  2. 5.0 μL 10x cloned Pfu buffer
  3. 0.4 μL dNTPs (25 mM each)
  4. 1.0 μL DNA template (100 ng/μL)
  5. 1.0 μL Primer 1 (ng/μL)
  6. 1.0 μL Primer 2 (ng/μL)
  7. 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)



Personal tools