User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/11/06: Difference between revisions

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==November 6, 2013==
==November 6, 2013==
===Procedures===
===Procedures===
 
*Further purification of ZnPPIX by procedure from Dr Hartings in "Purification of Laboratory Chemicals" By Wilfred L.F. Armarego and Christina Li Lin Chai
===Data===
**Hemin (ferriproptoporphyrin IX chloride)procedure used, substituting ZnPPIX for heme- calculations done to determine 1:1 molar ratio between ZnPPIX and acetic acid (AcOH)
 


===Notes===
===Notes===
 
*Initial ZnPPIX mixture made and filtration performed
*Tomorrow: filter off crystals from today's sample, washing with 50% aqueous AcOH (5mL), H2O (10mL), EtOH (2.5mL), and dried in air
*Next week: redissolve purified ZnPPIX in DMF, made samples, take UV-vis and fluorescence spectra for comparison to last week's (10/30/2013) spectra


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Revision as of 08:34, 6 November 2013

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November 6, 2013

Procedures

  • Further purification of ZnPPIX by procedure from Dr Hartings in "Purification of Laboratory Chemicals" By Wilfred L.F. Armarego and Christina Li Lin Chai
    • Hemin (ferriproptoporphyrin IX chloride)procedure used, substituting ZnPPIX for heme- calculations done to determine 1:1 molar ratio between ZnPPIX and acetic acid (AcOH)

Notes

  • Initial ZnPPIX mixture made and filtration performed
  • Tomorrow: filter off crystals from today's sample, washing with 50% aqueous AcOH (5mL), H2O (10mL), EtOH (2.5mL), and dried in air
  • Next week: redissolve purified ZnPPIX in DMF, made samples, take UV-vis and fluorescence spectra for comparison to last week's (10/30/2013) spectra


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