User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/23

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Line 13: Line 13:
*Run absorbance measurements
*Run absorbance measurements
*Use Beer's law to determine concentration
*Use Beer's law to determine concentration
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===Data===
+
===Data/Calculations===
*0.0026g ZnPPIX dissolved in 7mL DMF
*0.0026g ZnPPIX dissolved in 7mL DMF
*MW ZnPPIX: 626.032g/mol
*MW ZnPPIX: 626.032g/mol
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**assuming entire sample came off weigh boat
**assuming entire sample came off weigh boat
*Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM
*Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM
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===Notes===
+
**[[Image:2013_1023_ZnPPIX_beers_law_calcs.PNG]]
 +
*Absorbance Spectra and Literature Comparison:
 +
[[Image:2013_1023_ZnPPIX_abs_spectra_(mine).PNG]]<br.>
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[[Image:2013_1023_ZnPPIX_abs_spectra.PNG]]<br.>
 +
from "Metal and redox selectivity of protoporphyrin binding to the heme chaperone CcmE"

Revision as of 10:22, 23 October 2013

ZnPPIX Sample Prep Main project page
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October 23, 2013

Happy Mole Day!

Procedures

  • Dissolve filtered ZnPPIX from 10/16/2013 in DMF
  • Prepare several dilutions
  • Run absorbance measurements
  • Use Beer's law to determine concentration

Data/Calculations

  • 0.0026g ZnPPIX dissolved in 7mL DMF
  • MW ZnPPIX: 626.032g/mol
  • (0.0026g)×(1/626.032g/mol)×(1/0.007L)≈0.5933mM
    • assuming entire sample came off weigh boat
  • Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM
    • Image:2013_1023_ZnPPIX_beers_law_calcs.PNG
  • Absorbance Spectra and Literature Comparison:

Image:2013_1023_ZnPPIX_abs_spectra_(mine).PNG
Image:2013_1023_ZnPPIX_abs_spectra.PNG
from "Metal and redox selectivity of protoporphyrin binding to the heme chaperone CcmE"



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