User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/23: Difference between revisions
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*Run absorbance measurements | *Run absorbance measurements | ||
*Use Beer's law to determine concentration | *Use Beer's law to determine concentration | ||
===Data=== | ===Data/Calculations=== | ||
*0.0026g ZnPPIX dissolved in 7mL DMF | |||
*MW ZnPPIX: 626.032g/mol | |||
=== | *(0.0026g)×(1/626.032g/mol)×(1/0.007L)≈0.5933mM | ||
**assuming entire sample came off weigh boat | |||
*Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM | |||
**[[Image:2013_1023_ZnPPIX_beers_law_calcs.PNG]] | |||
====Absorbance Spectra and Literature Comparison:==== | |||
[[Image:2013_1023_ZnPPIX_abs_spectra_(mine).PNG]]<br.> | |||
[[Image:2013_1023_ZnPPIX_abs_spectra.PNG]]<br.> | |||
from "Metal and redox selectivity of protoporphyrin binding to the heme chaperone CcmE" | |||
Revision as of 07:23, 23 October 2013
ZnPPIX Sample Prep | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
October 23, 2013Happy Mole Day! Procedures
Data/Calculations
Absorbance Spectra and Literature Comparison:<br.> <br.> from "Metal and redox selectivity of protoporphyrin binding to the heme chaperone CcmE"
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