User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/23: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> ZnPPIX Sample Prep</span> | ||
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== | ==October 23, 2013== | ||
Happy Mole Day! | |||
===Procedures=== | ===Procedures=== | ||
*Dissolve filtered ZnPPIX from 10/16/2013 in DMF | |||
===Data=== | *Prepare several dilutions | ||
*Run absorbance measurements | |||
*Use Beer's law to determine concentration | |||
=== | ===Data/Calculations=== | ||
*0.0026g ZnPPIX dissolved in 7mL DMF | |||
*MW ZnPPIX: 626.032g/mol | |||
*(0.0026g)×(1/626.032g/mol)×(1/0.007L)≈0.5933mM | |||
**assuming entire sample came off weigh boat | |||
*Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM | |||
**[[Image:2013_1023_ZnPPIX_beers_law_calcs.PNG]] | |||
====Absorbance Spectra and Literature Comparison:==== | |||
[[Image:2013_1023_ZnPPIX_abs_spectra_(mine).PNG]]<br.> | |||
[[Image:2013_1023_ZnPPIX_abs_spectra.PNG]]<br.> | |||
from "Metal and redox selectivity of protoporphyrin binding to the heme chaperone CcmE" | |||
Revision as of 07:23, 23 October 2013
ZnPPIX Sample Prep | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
October 23, 2013Happy Mole Day! Procedures
Data/Calculations
Absorbance Spectra and Literature Comparison:<br.> <br.> from "Metal and redox selectivity of protoporphyrin binding to the heme chaperone CcmE"
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