User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/09

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(Procedures)
Line 12: Line 12:
*Procedure adapted from "On the preparation of metalloporphyrins"
*Procedure adapted from "On the preparation of metalloporphyrins"
**0.5g tetraphenylporphyrin refluxed in 50mL DMF
**0.5g tetraphenylporphyrin refluxed in 50mL DMF
-
**0.403g (stiochiometric amount) zinc acetate added, solution refluxed
+
**0.403g (stiochiometric amount) zinc acetate added, solution refluxed until reaction complete
 +
***completion determination by unchanged peaks in UV-vis measurements
**50mL distilled water added, solution chilled and then filtered
**50mL distilled water added, solution chilled and then filtered
***the filtrate was filtered over again and added to main filtered sample
***the filtrate was filtered over again and added to main filtered sample
 +
===Data===
===Data===
Absorbance Measurements
Absorbance Measurements

Revision as of 10:25, 16 October 2013

ZnPPIX Synthesis Main project page
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October 9, 2013

Procedures

  • Synthesize new ZnPPIX with Dr Hartings
  • Procedure adapted from "On the preparation of metalloporphyrins"
    • 0.5g tetraphenylporphyrin refluxed in 50mL DMF
    • 0.403g (stiochiometric amount) zinc acetate added, solution refluxed until reaction complete
      • completion determination by unchanged peaks in UV-vis measurements
    • 50mL distilled water added, solution chilled and then filtered
      • the filtrate was filtered over again and added to main filtered sample

Data

Absorbance Measurements

  • Used to determine reaction completion (based on the change in the peaks)

Image:2012 1009 ZnPPPIX abs.PNG

Notes

Next time:

  • Dissolve filtered ZnPPIX in DMF


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