User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/09: Difference between revisions
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*Procedure adapted from "On the preparation of metalloporphyrins" | *Procedure adapted from "On the preparation of metalloporphyrins" | ||
**0.5g tetraphenylporphyrin refluxed in 50mL DMF | **0.5g tetraphenylporphyrin refluxed in 50mL DMF | ||
**0.403g (stiochiometric amount) zinc acetate added, solution refluxed | **0.403g (stiochiometric amount) zinc acetate added, solution refluxed until reaction complete | ||
***completion determination by unchanged peaks in UV-vis measurements | |||
**50mL distilled water added, solution chilled and then filtered | **50mL distilled water added, solution chilled and then filtered | ||
***the filtrate was filtered over again and added to main filtered sample | ***the filtrate was filtered over again and added to main filtered sample | ||
===Data=== | ===Data=== | ||
Absorbance Measurements | Absorbance Measurements | ||
*Used to determine reaction completion (based on the change in the peaks) | *Used to determine reaction completion (based on the change in the peaks) | ||
[[Image:2012 1009 ZnPPPIX abs.PNG]] | [[Image:2012 1009 ZnPPPIX abs.PNG]] | ||
No visible change in peaks between 'ZnPPIX#2 100x' (dark blue line- the second sample of refluxing ZnPPIX taken) and 'ZnPPIX after' (light blue line- the final sample taken when reflux was stopped), used to determine that the reaction was complete.<br.> | |||
Peaks consistent with the literature: largest peak at ~500nm, next largest at ~600nm, smallest peak at ~550nm. | |||
===Notes=== | ===Notes=== |
Revision as of 08:31, 16 October 2013
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October 9, 2013Procedures
DataAbsorbance Measurements
No visible change in peaks between 'ZnPPIX#2 100x' (dark blue line- the second sample of refluxing ZnPPIX taken) and 'ZnPPIX after' (light blue line- the final sample taken when reflux was stopped), used to determine that the reaction was complete.<br.> Peaks consistent with the literature: largest peak at ~500nm, next largest at ~600nm, smallest peak at ~550nm. NotesNext time:
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