User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/09/04: Difference between revisions
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==September 4, 2013== | ==September 4, 2013== | ||
Zinc protophorphyrin (IX) sample preparation | Zinc protophorphyrin (IX) sample preparation | ||
===Replicating Fluorescence Samples from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/06/05 | ===Replicating Fluorescence Samples from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/06/05 06/05/2013]=== | ||
#2.22uM ZnPPIX | |||
#2.22uM ZnPPIX/1.11uM DNA-SH | |||
#1.934uM ZnPPIX/0.94uM DNA-SH/11.75uM AuNPs | |||
#2.22uM ZnPPIX/11.75uM AuNPs | |||
===Replicating Fluorescence from Publication=== | ===Replicating Fluorescence Procedure from Publication=== | ||
"Self Assembly of the ZnPPIX/G-Quadruplex Complex" in [http://pubs.acs.org/doi/abs/10.1021/ac300348v Fluorescence Detection of DNA, Adenosine-5′-Triphosphate (ATP), and Telomerase Activity by Zinc(II)-Protoporphyrin IX/G-Quadruplex Labels] | "Self Assembly of the ZnPPIX/G-Quadruplex Complex" in [http://pubs.acs.org/doi/abs/10.1021/ac300348v Fluorescence Detection of DNA, Adenosine-5′-Triphosphate (ATP), and Telomerase Activity by Zinc(II)-Protoporphyrin IX/G-Quadruplex Labels]<br.> | ||
#100uL 3uM 4-strand DNA- in place of telomerase solution | |||
#*100uL 50mM KCl and 5uM ZnPPIX (to ensure excess for 1 G-quadruplex formation) | |||
#*Allow to react for 10 minutes | |||
#100uL 3uM 8-strand DNA- in place of telomerase solution | |||
#*100uL 50mM KCl and 10uM ZnPPIX (to ensure excess for 2 G-quadruplex formations) | |||
#*Allow to react from 10 minutes | |||
===Modifying Fluorescence Samples from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/06/05 | ===Modifying Fluorescence Samples from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/06/05 06/05/2013]=== | ||
#2.22uM ZnPPIX/1.11uM 4-strand DNA (to ensure excess for 1 G-quadruplex formation) | |||
#3.33uM ZnPPIX/1.11uM 8-strand DNA (to ensure excess for 2 G-quadruplex formations) | |||
===Fluorescence Spectra=== | |||
[[Image:2013_0904_ZnPPIX_fluor.PNG]] | |||
===Notes=== | |||
*No signal observed for any of the samples. The raw data was found to be exactly the same for each sample. UV-vis and fluorescence measurements will be taken again next time. | |||
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Revision as of 07:28, 4 December 2013
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September 4, 2013Zinc protophorphyrin (IX) sample preparation Replicating Fluorescence Samples from 06/05/2013
Replicating Fluorescence Procedure from Publication"Self Assembly of the ZnPPIX/G-Quadruplex Complex" in Fluorescence Detection of DNA, Adenosine-5′-Triphosphate (ATP), and Telomerase Activity by Zinc(II)-Protoporphyrin IX/G-Quadruplex Labels<br.>
Modifying Fluorescence Samples from 06/05/2013
Fluorescence SpectraNotes
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