User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/12: Difference between revisions
From OpenWetWare
Line 40: | Line 40: | ||
***To prepare 10mM MHA solution: | ***To prepare 10mM MHA solution: | ||
***(5 mL)×(L/1000mL)×(10mmoles/L)×(1mol/1000mmoles)×(288.49g/mol)=0.0144g MHA in 5mL 2-propanol | ***(5 mL)×(L/1000mL)×(10mmoles/L)×(1mol/1000mmoles)×(288.49g/mol)=0.0144g MHA in 5mL 2-propanol | ||
***To prepare 5mL of 9.4nM AuNPs: | |||
***(20.67nM)V=(9.4nM)(5mL) | |||
***V=1.584mL 20.67nM AuNPs in 3.416mL PBS buffer | |||
***Combine MHA and AuNP solutions | |||
*Day Two: | *Day Two: | ||
**Prepare the following solutions: | **Prepare the following solutions: |
Revision as of 07:44, 19 July 2013
Meeting, DNA/AuNP Attachment | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
July 12, 2013Notes from Meeting
DNA and AuNP Attachment ProcedureCalculations
NotesProcedure stopped because the nanoparticles could not be extracted by centrifugation. Absorbance data to be taken tomorrow. Procedure Update: Silica-coating AuNPs by MHAWe decided to follow the MHA procedure because MHA reduces the number of gold cores per silica-coated particles, and we would ideally have each individual gold nanoparticle covered by a layer of silica. Using the MHA versus not using it has also been very effective in the trials conducted so far, and we think that using MHA will allow us to reach our desired optimal shell thickness of approximately 35nm.<br.>
|