User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/12: Difference between revisions
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===DNA and AuNP Attachment Procedure=== | ===DNA and AuNP Attachment Procedure=== | ||
====Calculations==== | ====Calculations==== | ||
Done by [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/12 Allison Alix].<br.> | *Done by [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/12 Allison Alix].<br.> | ||
Combine 231.3uL | *Extraction Procedure: | ||
**Combine 250uL 4.45uM DNA (diluted in PBS) with 250uL 100nM DTT in 4% TEA | |||
**React for 10 minutes | |||
**Extract DTT with 4 2mL aliquots of ethyl acetate (DTT will partition into ethyl acetate, DNA will partition into aqueous solution) | |||
*Combine 231.3uL 2.226uM DNA, 231.3uL 29.67nM 14.7nm AuNPs, and 37.3uL sodium citrate buffer prepared 07/11/2013 <br.> | |||
**Final Concentrations in Solution: | |||
***DNA: 1.03uM | |||
***AuNP: 13.73nM | |||
***Sodium citrate: 20mM | |||
*React for 10 minutes | |||
*Centrifuge 4 times, extracting the supernatant each time and then redispersing in 250uL 50nM HEPES buffer | |||
Revision as of 08:52, 12 July 2013
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July 12, 2013Notes from Meeting
DNA and AuNP Attachment ProcedureCalculations
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