User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/12: Difference between revisions
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*Centrifuge 4 times, extracting the supernatant each time and then redispersing in 250uL 50nM HEPES buffer | *Centrifuge 4 times, extracting the supernatant each time and then redispersing in 250uL 50nM HEPES buffer | ||
*Take absorbance of final solution and three supernatants | *Take absorbance of final solution and three supernatants | ||
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Procedure stopped because the nanoparticles could not be extracted by centrifugation. Absorbance data to be taken tomorrow. | |||
===Procedure Update: Silica-coating AuNPs by MHA=== | ===Procedure Update: Silica-coating AuNPs by MHA=== |
Revision as of 11:31, 12 July 2013
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July 12, 2013Notes from Meeting
DNA and AuNP Attachment ProcedureCalculations
NotesProcedure stopped because the nanoparticles could not be extracted by centrifugation. Absorbance data to be taken tomorrow. Procedure Update: Silica-coating AuNPs by MHAWe decided to follow the MHA procedure because MHA reduces the number of gold cores per silica-coated particles, and we would ideally have each individual gold nanoparticle covered by a layer of silica. Using the MHA versus not using it has also been very effective in the trials conducted so far, and we think that using MHA will allow us to reach our desired optimal shell thickness of approximately 35nm.<br.>
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