User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/12: Difference between revisions
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**Stir solutions for one hour to allow reaction to proceed | **Stir solutions for one hour to allow reaction to proceed | ||
**Centrifuge solutions at 4000 rpm for one hour | **Centrifuge solutions at 4000 rpm for one hour | ||
**Re-suspend particles in | **Re-suspend particles in PBS to a final concentration of 5nM? | ||
Revision as of 11:00, 12 July 2013
Meeting, DNA/AuNP Attachment | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
July 12, 2013Notes from Meeting
DNA and AuNP Attachment ProcedureCalculations
Absorbance DataProcedure Update: Silica-coating AuNPs by MHAWe decided to follow the MHA procedure because MHA reduces the number of gold cores per silica-coated particles, and we would ideally have each individual gold nanoparticle covered by a layer of silica. Using the MHA versus not using it has also been very effective in the trials conducted so far. We also think that using MHA will allow us to reach our desired optimal shell thickness of approximately 35nm.<br.>
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