User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/12

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(July 12, 2013)
(July 12, 2013)
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==July 12, 2013==
==July 12, 2013==
===Notes from Meeting===
===Notes from Meeting===
 +
*Adjust range and use SymphoTime to better fit FCS graphs in Igor
 +
*Use error bars on calibration graphs (for Igor <N> values)
 +
*Focus on attaching AuNPs to DNA & hybridizing MB to run on FCS
 +
*Use a range of volumes of TES in MHA silica-coating procedure to change silica-coating thickness
 +
**Take absorbance data to see differences between solution, then use TEM imaging to verify presence of silica-coating
===DNA and AuNP Attachment Procedure===
===DNA and AuNP Attachment Procedure===
====Calculations====
====Calculations====
-
Done by [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/12 Allison Alix].
+
Done by [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/07/12 Allison Alix].<br.>
 +
Combine 231.3uL 1.03uM DNA, 231.3uL 29.67nM 14.7nm AuNPs, and 37.3uL sodium citrate buffer prepared 07/11/2013 <br.>

Revision as of 11:39, 12 July 2013

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July 12, 2013

Notes from Meeting

  • Adjust range and use SymphoTime to better fit FCS graphs in Igor
  • Use error bars on calibration graphs (for Igor <N> values)
  • Focus on attaching AuNPs to DNA & hybridizing MB to run on FCS
  • Use a range of volumes of TES in MHA silica-coating procedure to change silica-coating thickness
    • Take absorbance data to see differences between solution, then use TEM imaging to verify presence of silica-coating

DNA and AuNP Attachment Procedure

Calculations

Done by Allison Alix.
Combine 231.3uL 1.03uM DNA, 231.3uL 29.67nM 14.7nm AuNPs, and 37.3uL sodium citrate buffer prepared 07/11/2013



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