User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/11: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> FCS Measurements</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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**Run three times, 90s each
**Run three times, 90s each
**Used to properly align laser
**Used to properly align laser
**Vortexed before running
*DNA/MB
*DNA/MB
**600pM DNA/500pM MB
**600pM DNA/500pM MB
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**600pM DNA/100pM MB
**600pM DNA/100pM MB
**Run three times, 500s each
**Run three times, 500s each
**All samples heated to ~70°C for 25min, cooled for at least 20min, and vortexed before running
===FCS Measurements===
===FCS Measurements===
====From samples run 07/10/2013====
====From samples run 07/10/2013====
 
[[Image:2013_0710_OD_both_compliations.PNG]]
====From samples run 07/11/2013====
====From samples run 07/11/2013====
 
[[Image:2013_0711_PBS_compliation.PNG]]<br.>
<br.>
[[Image:2013_0711 all DNA-MB.PNG]]<br.>


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Latest revision as of 23:03, 26 September 2017

FCS Measurements Main project page
Previous entry      Next entry

July 11, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted
    • Run three times, 90s each
    • Used to properly align laser
    • Vortexed before running
  • DNA/MB
    • 600pM DNA/500pM MB
    • 600pM DNA/400pM MB
    • 600pM DNA/300pM MB
    • 600pM DNA/200pM MB
    • 600pM DNA/100pM MB
    • Run three times, 500s each
    • All samples heated to ~70°C for 25min, cooled for at least 20min, and vortexed before running

FCS Measurements

From samples run 07/10/2013

From samples run 07/11/2013

<br.> <br.> <br.>


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