User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/11: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> FCS Measurements</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==July 11, 2013== | ||
* | ===FCS Sample Prep=== | ||
*Green fluorescent beads | |||
**10000x diluted | |||
**Run three times, 90s each | |||
**Used to properly align laser | |||
**Vortexed before running | |||
*DNA/MB | |||
**600pM DNA/500pM MB | |||
**600pM DNA/400pM MB | |||
**600pM DNA/300pM MB | |||
**600pM DNA/200pM MB | |||
**600pM DNA/100pM MB | |||
**Run three times, 500s each | |||
**All samples heated to ~70°C for 25min, cooled for at least 20min, and vortexed before running | |||
===FCS Measurements=== | |||
====From samples run 07/10/2013==== | |||
[[Image:2013_0710_OD_both_compliations.PNG]] | |||
====From samples run 07/11/2013==== | |||
[[Image:2013_0711_PBS_compliation.PNG]]<br.> | |||
<br.> | |||
[[Image:2013_0711 all DNA-MB.PNG]]<br.> | |||
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Latest revision as of 23:03, 26 September 2017
FCS Measurements | Main project page Previous entry Next entry |
July 11, 2013FCS Sample Prep
FCS MeasurementsFrom samples run 07/10/2013From samples run 07/11/2013 |