User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/11: Difference between revisions

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**Run three times, 90s each
**Run three times, 90s each
**Used to properly align laser
**Used to properly align laser
**Vortexed before running
*DNA/MB
*DNA/MB
**600pM DNA/500pM MB
**600pM DNA/500pM MB
Line 19: Line 20:
**600pM DNA/100pM MB
**600pM DNA/100pM MB
**Run three times, 500s each
**Run three times, 500s each
**All samples heated to ~70°C for 25min, cooled for at least 20min, and vortexed before running
===FCS Measurements===
===FCS Measurements===
====From samples run 07/10/2013====
====From samples run 07/10/2013====
 
[[Image:2013_0710_OD_both_compliations.PNG]]
====From samples run 07/11/2013====
====From samples run 07/11/2013====
 
[[Image:2013_0711_PBS_compliation.PNG]]<br.>
<br.>
[[Image:2013_0711 all DNA-MB.PNG]]<br.>


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Revision as of 11:50, 15 July 2013

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July 11, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted
    • Run three times, 90s each
    • Used to properly align laser
    • Vortexed before running
  • DNA/MB
    • 600pM DNA/500pM MB
    • 600pM DNA/400pM MB
    • 600pM DNA/300pM MB
    • 600pM DNA/200pM MB
    • 600pM DNA/100pM MB
    • Run three times, 500s each
    • All samples heated to ~70°C for 25min, cooled for at least 20min, and vortexed before running

FCS Measurements

From samples run 07/10/2013

From samples run 07/11/2013

<br.> <br.> <br.>


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