User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/11

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(Autocreate 2013/07/11 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence)
Current revision (14:50, 15 July 2013) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> FCS Measurements</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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==July 11, 2013==
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* Insert content here...
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===FCS Sample Prep===
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*Green fluorescent beads
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**10000x diluted
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**Run three times, 90s each
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**Used to properly align laser
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**Vortexed before running
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*DNA/MB
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**600pM DNA/500pM MB
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**600pM DNA/400pM MB
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**600pM DNA/300pM MB
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**600pM DNA/200pM MB
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**600pM DNA/100pM MB
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**Run three times, 500s each
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**All samples heated to ~70°C for 25min, cooled for at least 20min, and vortexed before running
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===FCS Measurements===
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====From samples run 07/10/2013====
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[[Image:2013_0710_OD_both_compliations.PNG]]
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====From samples run 07/11/2013====
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[[Image:2013_0711_PBS_compliation.PNG]]<br.>
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<br.>
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[[Image:2013_0711 all DNA-MB.PNG]]<br.>
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Current revision

FCS Measurements Main project page
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July 11, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted
    • Run three times, 90s each
    • Used to properly align laser
    • Vortexed before running
  • DNA/MB
    • 600pM DNA/500pM MB
    • 600pM DNA/400pM MB
    • 600pM DNA/300pM MB
    • 600pM DNA/200pM MB
    • 600pM DNA/100pM MB
    • Run three times, 500s each
    • All samples heated to ~70°C for 25min, cooled for at least 20min, and vortexed before running

FCS Measurements

From samples run 07/10/2013

Image:2013_0710_OD_both_compliations.PNG

From samples run 07/11/2013

Image:2013_0711_PBS_compliation.PNG

Image:2013_0711 all DNA-MB.PNG


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