User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/10

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> FCS Measurements</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==July 10, 2013==
==July 10, 2013==
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===DNA/AuNP Attachement===
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*Extraction procedure from Allison Alix on [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/05/31 05/31/2013]
===FCS Sample Prep===
===FCS Sample Prep===
*Green fluorescent beads
*Green fluorescent beads
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**50pM
**50pM
**Run three times, 500s per sample
**Run three times, 500s per sample
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===FCS Measurements===
 

Current revision

FCS Measurements Main project page
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July 10, 2013

DNA/AuNP Attachement

  • Extraction procedure from Allison Alix on 05/31/2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted
    • Run three times, 90s per sample
  • Oligo D
    • 200pM
    • 175pM
    • 150pM
    • 125pM
    • 100pM
    • 75pM
    • 50pM
    • Run three times, 500s per sample



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