User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/08: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Meeting, FCS Measurements</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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*Look up AuNP/DNA/MB fluorescent data and run samples/make and run new samples | *Look up AuNP/DNA/MB fluorescent data and run samples/make and run new samples | ||
*Look in literature for ways to calculate silica shell thickness without TEM results | *Look in literature for ways to calculate silica shell thickness without TEM results | ||
===FCS Sample Prep=== | |||
*Green fluorescent beads | |||
**10000x diluted | |||
**Run 3x, 90s | |||
*Oligo D | |||
**150pM | |||
**125pM | |||
**100pM | |||
**Run 3x, 500s | |||
*DNA/MB: DNA held at a constant concentration to isolate background from DNA | |||
**600/500 | |||
**600/400 | |||
**600/300 | |||
**600/200 | |||
**600/100 | |||
**Run 3x, 500s | |||
Revision as of 06:55, 10 July 2013
Meeting, FCS Measurements | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
July 8, 2013Notes from Meeting
FCS Sample Prep
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