User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/08

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(July 8, 2013)
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==July 8, 2013==
==July 8, 2013==
===Notes from Meeting===
===Notes from Meeting===
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*
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*Since we're seeing some noise from just PBS on the FCS: make new PBS and run, parse out 10mL samples to avoid contaimination
 +
*Sonicate or centrifuge green fluorescent beads before running to prevent aggregation while measurements are being taken
 +
*Go up to 500pM for Oligo D samples (and lower concentrations)
 +
*Use a high, constant concentration of DNA with lower, decreasing concentration of MB
 +
*Check for consistent diffusion times between samples run on different days
 +
**Different diffusion times indicate background is something other than unbound DNA
 +
*Look up AuNP/DNA/MB fluorescent data and run samples/make and run new samples

Revision as of 08:58, 8 July 2013

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July 8, 2013

Notes from Meeting

  • Since we're seeing some noise from just PBS on the FCS: make new PBS and run, parse out 10mL samples to avoid contaimination
  • Sonicate or centrifuge green fluorescent beads before running to prevent aggregation while measurements are being taken
  • Go up to 500pM for Oligo D samples (and lower concentrations)
  • Use a high, constant concentration of DNA with lower, decreasing concentration of MB
  • Check for consistent diffusion times between samples run on different days
    • Different diffusion times indicate background is something other than unbound DNA
  • Look up AuNP/DNA/MB fluorescent data and run samples/make and run new samples



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