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 FCS Measurements
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July 5, 2013
FCS Sample Prep
- Green fluorescent beads
- 10000x diluted, run 3x for 90s
- PBS
- To check for background, run 3x for 200s
- DNA/MB
- 125/100pM, run 3x for 500s
- 100/80pM, run 3x for 500s
- 75/60pM, run 3x for 500s
- 50/40pM, run 3x for 500s
- 25/20pM, run 3x for 500s
FCS Data
Graphs generated using Igor Pro.<br.>
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10000x diluted green fluorescent beads were used to align the laser. Sample was run three times, for 90s each.<br.>
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PBS run to check for background fluorescence. Sample was run three times, 200s each to eliminate noise.<br.>
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Notes
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FCS Measurements
