User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/05: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> FCS Measurements</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> FCS Measurements</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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**25/20pM, run 3x for 500s | **25/20pM, run 3x for 500s | ||
===FCS Data=== | ===FCS Data=== | ||
Graphs generated using Igor Pro.<br.> | |||
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[[Image:Avg_Beads.png]]<br.> | |||
10000x diluted green fluorescent beads were used to align the laser. Sample was run three times, for 90s each.<br.> | |||
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[[Image:Avg_PBS.png]]<br.> | |||
PBS run to check for background fluorescence. Sample was run three times, 200s each to eliminate noise.<br.> | |||
[[Image:Avg_125pM.png]]<br.> | |||
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[[Image:Avg_100pM.png]]<br.> | |||
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[[Image:Avg_75pM.png]]<br.> | |||
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[[Image:Avg_50pM.png]]<br.> | |||
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[[Image:Avg_25pM.png]]<br.> | |||
All DNA/MB samples run three times, for 500s.<br.> | |||
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Calibration Graph:<br.> | |||
[[Image:2013_0705_calibration.PNG]] | |||
Latest revision as of 22:58, 26 September 2017
FCS Measurements | Main project page Previous entry Next entry |
July 5, 2013FCS Sample Prep
FCS DataGraphs generated using Igor Pro.<br.> <br.> <br.> 10000x diluted green fluorescent beads were used to align the laser. Sample was run three times, for 90s each.<br.> <br.> <br.> PBS run to check for background fluorescence. Sample was run three times, 200s each to eliminate noise.<br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> <br.> All DNA/MB samples run three times, for 500s.<br.> <br.> Calibration Graph:<br.>
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