User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/05

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> FCS Measurements</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==July 5, 2013==
==July 5, 2013==
===FCS Sample Prep===
===FCS Sample Prep===
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*Green fluorescent beads
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**10000x diluted, run 3x for 90s
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*PBS
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**To check for background, run 3x for 200s
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*DNA/MB
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**125/100pM, run 3x for 500s
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**100/80pM, run 3x for 500s
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**75/60pM, run 3x for 500s
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**50/40pM, run 3x for 500s
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**25/20pM, run 3x for 500s
===FCS Data===
===FCS Data===
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====Notes====
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Graphs generated using Igor Pro.<br.>
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<br.>
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[[Image:Avg_Beads.png]]<br.>
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10000x diluted green fluorescent beads were used to align the laser.  Sample was run three times, for 90s each.<br.>
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<br.>
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[[Image:Avg_PBS.png]]<br.>
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PBS run to check for background fluorescence.  Sample was run three times, 200s each to eliminate noise.<br.>
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[[Image:Avg_125pM.png]]<br.>
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<br.>
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[[Image:Avg_100pM.png]]<br.>
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<br.>
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[[Image:Avg_75pM.png]]<br.>
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<br.>
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[[Image:Avg_50pM.png]]<br.>
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<br.>
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[[Image:Avg_25pM.png]]<br.>
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All DNA/MB samples run three times, for 500s.<br.>
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<br.>
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Calibration Graph:<br.>
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[[Image:2013_0705_calibration.PNG]]

Current revision

FCS Measurements Main project page
Previous entry      Next entry

July 5, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted, run 3x for 90s
  • PBS
    • To check for background, run 3x for 200s
  • DNA/MB
    • 125/100pM, run 3x for 500s
    • 100/80pM, run 3x for 500s
    • 75/60pM, run 3x for 500s
    • 50/40pM, run 3x for 500s
    • 25/20pM, run 3x for 500s

FCS Data

Graphs generated using Igor Pro.

Image:Avg_Beads.png
10000x diluted green fluorescent beads were used to align the laser. Sample was run three times, for 90s each.

Image:Avg_PBS.png
PBS run to check for background fluorescence. Sample was run three times, 200s each to eliminate noise.
Image:Avg_125pM.png

Image:Avg_100pM.png

Image:Avg_75pM.png

Image:Avg_50pM.png

Image:Avg_25pM.png
All DNA/MB samples run three times, for 500s.

Calibration Graph:
Image:2013_0705_calibration.PNG



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