User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/05

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(July 5, 2013)
(July 5, 2013)
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**10000x diluted, run 3x for 90s
**10000x diluted, run 3x for 90s
*PBS
*PBS
-
**To check for background, run 3x for 90s
+
**To check for background, run 3x for 200s
*DNA/MB
*DNA/MB
**125/100pM, run 3x for 500s
**125/100pM, run 3x for 500s

Revision as of 14:12, 5 July 2013

FCS Measurements Main project page
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July 5, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted, run 3x for 90s
  • PBS
    • To check for background, run 3x for 200s
  • DNA/MB
    • 125/100pM, run 3x for 500s
    • 100/80pM, run 3x for 500s
    • 75/60pM, run 3x for 500s
    • 50/40pM, run 3x for 500s
    • 25/20pM, run 3x for 500s

FCS Data

Notes


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