User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/05: Difference between revisions

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===FCS Sample Prep===
===FCS Sample Prep===
*Green fluorescent beads
*Green fluorescent beads
**10000x diluted
**10000x diluted, run 3x for 90s
*PBS
**To check for background, run 3x for 90s
*DNA/MB
*DNA/MB
**125/100pM
**125/100pM, run 3x for 500s
**100/80pM
**100/80pM, run 3x for 500s
**75/60pM
**75/60pM, run 3x for 500s
**50/40pM
**50/40pM, run 3x for 500s
**25/20pM
**25/20pM, run 3x for 500s
===FCS Data===
===FCS Data===
====Notes====
====Notes====

Revision as of 06:48, 5 July 2013

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July 5, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted, run 3x for 90s
  • PBS
    • To check for background, run 3x for 90s
  • DNA/MB
    • 125/100pM, run 3x for 500s
    • 100/80pM, run 3x for 500s
    • 75/60pM, run 3x for 500s
    • 50/40pM, run 3x for 500s
    • 25/20pM, run 3x for 500s

FCS Data

Notes


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