User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/02
From OpenWetWare
FCS Measurements | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
July 2, 2013FCS Sample Prep
FCS Data<br.> Beads used to align laser; three samples run at 90s each, average values used. Curve turned out as expected.<br.> <br.> Oligo D: three samples run at each concentration, 500s each, average values used. Y-intercepts increase as concentration decreases, as expected.<br.> <br.> DNA/MB: three samples run at each concentration, 500s each, average values used. Y-intercepts increase as concentration decreases until 50pM. DNA in excess rather than MB to ensure binding.<br.> <br.> Calibration graphs for Oligo D and DNA/MB samples. Oligo D trend turned out as expected; DNA/MB trend dropped at 50pM concentration. DNA/MB samples will be remade and run again tomorrow. <br.> |