User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/01: Difference between revisions
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**25pM/12.5pM: from 2nM/1.6nM dilutions | **25pM/12.5pM: from 2nM/1.6nM dilutions | ||
===FCS Data=== | ===FCS Data=== | ||
[[Image:FCS_data_2013_0701_OD%2C_MB-DNA_calibrations.PNG]] | |||
====Notes===== | |||
*Calibration curves did not turn out as expected (linear trend) | |||
*DNA, not MB, should be in excess for DNA-MB samples to ensure all MB is bound | |||
*Focus on three concentrations, run each sample several times, average curves | |||
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Revision as of 13:07, 1 July 2013
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July 1, 2013FCS Sample Preparation
FCS DataNotes=
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