User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/01: Difference between revisions
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**50pM/40pM: from 2nM/1.6nM dilutions | **50pM/40pM: from 2nM/1.6nM dilutions | ||
**25pM/12.5pM: from 2nM/1.6nM dilutions | **25pM/12.5pM: from 2nM/1.6nM dilutions | ||
===FCS Data=== | ===FCS Data: Calibration Curves=== | ||
[[Image:FCS_data_2013_0701_OD%2C_MB-DNA_calibrations.PNG]] | [[Image:FCS_data_2013_0701_OD%2C_MB-DNA_calibrations.PNG]] | ||
====Notes==== | ====Notes==== | ||
*Use green fluorescent beads to align laser | |||
*Calibration curves did not turn out as expected (linear trend) | *Calibration curves did not turn out as expected (linear trend) | ||
*DNA, not MB, should be in excess for DNA-MB samples to ensure all MB is bound | *DNA, not MB, should be in excess for DNA-MB samples to ensure all MB is bound | ||
*Focus on three concentrations, run each sample several times, average curves | *Focus on three concentrations, run each sample several times, average curves | ||
**Oligo D: 150, 125, 100pM run three times at 500s per sample | |||
**DNA/MB: 100pM DNA/80pM MB, 75/60, 50/40pM run three times at 500s per sample (increase to 700s per sample if spectra are noisey) | |||
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Revision as of 13:12, 1 July 2013
 FCS Measurements
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July 1, 2013FCS Sample Preparation
FCS Data: Calibration Curves
Notes
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