User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/28: Difference between revisions
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**100, 80, 60, 20pM Oligo D | **100, 80, 60, 20pM Oligo D | ||
**10, 1, 5nM, 500, 100, 50, 25pM DNA/MB samples | **10, 1, 5nM, 500, 100, 50, 25pM DNA/MB samples | ||
====Results==== | ====Results and Notes==== | ||
Calibration graphs:<br.> | |||
[[Image:FCS_data_2013_0628_calibration_graphs.PNG]]<br.> | |||
<br.> | |||
*Oligo D samples were run for 300s, the 60pM and 80pM samples are still inconsistent with the trend of the other concentrations | |||
*The first round of MB/DNA samples were run for 300s, results had lots of noise and inconsistencies | |||
*The second round of MB/DNA samples were run for 700s to eliminate noise, only the 25pM sample lied outside the trend of the other concentrations | |||
*Future work: | |||
**Always align laser with 10000x diluted green fluorescent beads, include spectra for comparison | |||
**Create more samples for MB/DNA with 100pM intervals from 1nM to 100pM, and a 75pM sample | |||
**Create more samples for Oligo D at 50, 30, and 10pM concentrations | |||
**For pM concentrations increase time to 700s to eliminate noise from spectra | |||
**Attach AuNPs in proper ratio at successfully run concentrations of MB/DNA | |||
Revision as of 13:08, 28 June 2013
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June 28, 2013Notes from Meeting
FCS Measurements
Results and NotesCalibration graphs:<br.> <br.> <br.>
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