User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27: Difference between revisions
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===FCS Measurements=== | ===FCS Measurements=== | ||
*Samples were prepared again for the Oligo D and DNA/MB | *Samples were prepared again for the Oligo D and DNA/MB | ||
Revision as of 13:31, 28 June 2013
Fluorescence and FCS Data | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
June 27, 2013DNA/ThT Samples for Calibration Graph
ResultsInconsistent Spectra<br.> <br.> <br.> <br.> <br.> <br.> NotesWe still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency. FCS Measurements
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