User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence and FCS Data</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
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===Notes===
====Notes====
We still cannot produce consistent spectra for concentrations about 500nM.  This may be due to a limit of bonding: only a certain about of ThT molecules can bind to a give number of DNA molecules and that number has been reached, making higher concentrations irrelevant.
We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
===FCS Measurements===
*Samples were prepared again for the Oligo D and DNA/MB


   
   

Revision as of 13:31, 28 June 2013

Fluorescence and FCS Data <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

June 27, 2013

DNA/ThT Samples for Calibration Graph

  • Remade samples at 500, 600, 700, 800, 900nM
  • Fluorescence measurements taken

Results

<br.> <br.> <br.> <br.>

Inconsistent Spectra

<br.> <br.> <br.> <br.> <br.> <br.>

Notes

We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.

FCS Measurements

  • Samples were prepared again for the Oligo D and DNA/MB




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