User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27
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June 27, 2013
DNA/ThT Samples for Calibration Graph
We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.