User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/06/27 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence)
(Notes)
(2 intermediate revisions not shown.)
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
+
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence and FCS Data</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Entry title==
+
==June 27, 2013==
-
* Insert content here...
+
===DNA/ThT Samples for Calibration Graph===
 +
*Remade samples at 500, 600, 700, 800, 900nM
 +
*Fluorescence measurements taken
 +
====Results====
 +
[[Image:Fluor_data_DNA_ThT_compliation.png]]<br.>
 +
<br.>
 +
[[Image:Fluor_data_DNA_ThT_compliation_calibration.png]]<br.>
 +
<br.>
 +
=====Inconsistent Spectra=====
 +
[[Image:Fluor_data_600nM_DNA_comparison.png]]<br.>
 +
<br.>
 +
[[Image:Fluor_data_700nM_DNA_comparison.png]]<br.>
 +
<br.>
 +
[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
 +
<br.>
 +
===Notes===
 +
We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
 +
 
 +

Revision as of 11:41, 27 June 2013

Fluorescence and FCS Data Main project page
Previous entry      Next entry

June 27, 2013

DNA/ThT Samples for Calibration Graph

  • Remade samples at 500, 600, 700, 800, 900nM
  • Fluorescence measurements taken

Results

Image:Fluor_data_DNA_ThT_compliation.png

Image:Fluor_data_DNA_ThT_compliation_calibration.png

Inconsistent Spectra

Image:Fluor_data_600nM_DNA_comparison.png

Image:Fluor_data_700nM_DNA_comparison.png

Image:Fluor_data_800nM_DNA_comparison.png

Notes

We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.




Personal tools