User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27

From OpenWetWare

< User:Madeleine Y. Bee | Notebook | Single Molecule Fluorescence | 2013 | 06(Difference between revisions)
Jump to: navigation, search
(Notes)
Current revision (16:31, 28 June 2013) (view source)
(FCS Measurements)
 
(One intermediate revision not shown.)
Line 22: Line 22:
[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
<br.>
<br.>
-
===Notes===
+
====Notes====
We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
 +
===FCS Measurements===
 +
*Samples were prepared again for the Oligo D and DNA/MB
   
   

Current revision

Fluorescence and FCS Data Main project page
Previous entry      Next entry

June 27, 2013

DNA/ThT Samples for Calibration Graph

  • Remade samples at 500, 600, 700, 800, 900nM
  • Fluorescence measurements taken

Results

Image:Fluor_data_DNA_ThT_compliation.png

Image:Fluor_data_DNA_ThT_compliation_calibration.png

Inconsistent Spectra

Image:Fluor_data_600nM_DNA_comparison.png

Image:Fluor_data_700nM_DNA_comparison.png

Image:Fluor_data_800nM_DNA_comparison.png

Notes

We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.

FCS Measurements

  • Samples were prepared again for the Oligo D and DNA/MB




Personal tools