User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence and FCS Data</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence and FCS Data</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
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===Notes===
====Notes====
We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
===FCS Measurements===
*Samples were prepared again for the Oligo D and DNA/MB


   
   

Latest revision as of 22:48, 26 September 2017

Fluorescence and FCS Data Main project page
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June 27, 2013

DNA/ThT Samples for Calibration Graph

  • Remade samples at 500, 600, 700, 800, 900nM
  • Fluorescence measurements taken

Results

<br.> <br.> <br.> <br.>

Inconsistent Spectra

<br.> <br.> <br.> <br.> <br.> <br.>

Notes

We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.

FCS Measurements

  • Samples were prepared again for the Oligo D and DNA/MB




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