User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence and FCS Data</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Fluorescence and FCS Data</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.> | [[Image:Fluor_data_800nM_DNA_comparison.png]]<br.> | ||
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===Notes=== | ====Notes==== | ||
We still cannot produce consistent spectra for concentrations | We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency. | ||
===FCS Measurements=== | |||
*Samples were prepared again for the Oligo D and DNA/MB | |||
Latest revision as of 22:48, 26 September 2017
Fluorescence and FCS Data | Main project page Previous entry Next entry |
June 27, 2013DNA/ThT Samples for Calibration Graph
ResultsInconsistent Spectra<br.> <br.> <br.> <br.> <br.> <br.> NotesWe still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency. FCS Measurements
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