User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27

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(FCS Measurements)
 
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[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
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===Notes===
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====Notes====
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We still cannot produce consistent spectra for concentrations about 500nM.  This may be due to a limit of bonding: only a certain about of ThT molecules can bind to a give number of DNA molecules and that number has been reached, making higher concentrations irrelevant.
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We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
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===FCS Measurements===
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*Samples were prepared again for the Oligo D and DNA/MB
   
   

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Fluorescence and FCS Data Main project page
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June 27, 2013

DNA/ThT Samples for Calibration Graph

  • Remade samples at 500, 600, 700, 800, 900nM
  • Fluorescence measurements taken

Results

Image:Fluor_data_DNA_ThT_compliation.png

Image:Fluor_data_DNA_ThT_compliation_calibration.png

Inconsistent Spectra

Image:Fluor_data_600nM_DNA_comparison.png

Image:Fluor_data_700nM_DNA_comparison.png

Image:Fluor_data_800nM_DNA_comparison.png

Notes

We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.

FCS Measurements

  • Samples were prepared again for the Oligo D and DNA/MB




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