User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/04/23

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(April 23, 2013)
Current revision (12:14, 24 April 2013) (view source)
(April 23, 2013)
 
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=====Preparation of Gel and Samples=====
=====Preparation of Gel and Samples=====
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Procedure from Allison Alix on 2013/03/29 [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/03/29]
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Procedure from Allison Alix on [[http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/03/29| 2013/03/29]]
=====Attached AuNP to thiol DNA with ThT=====
=====Attached AuNP to thiol DNA with ThT=====
Combine 250μL DNA/ThT solution with 250μL 4% TEA 100mM DNA<br.>
Combine 250μL DNA/ThT solution with 250μL 4% TEA 100mM DNA<br.>
Allow solution to react for 10 minutes<br.>
Allow solution to react for 10 minutes<br.>
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Follow same extraction procedure from March 19, 2013 [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/03/19]
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Follow same extraction procedure from [[http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/03/19| March 19, 2013]]

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April 23, 2013

Absorbance and Florescence Data of thiol DNA and ThT

Image:4.23.13_absorbance_DNA_and_Tht.png

Image:4.23.13_florescence_DNA_and_ThT.png

Preparation of Gel and Samples

Procedure from Allison Alix on [2013/03/29]

Attached AuNP to thiol DNA with ThT

Combine 250μL DNA/ThT solution with 250μL 4% TEA 100mM DNA
Allow solution to react for 10 minutes
Follow same extraction procedure from [March 19, 2013]



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