User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/04/23: Difference between revisions
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=====Preparation of Gel and Samples===== | =====Preparation of Gel and Samples===== | ||
Procedure from Allison Alix on | Procedure from Allison Alix on [[http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/03/29| 2013/03/29]] | ||
=====Attached AuNP to thiol DNA with ThT===== | =====Attached AuNP to thiol DNA with ThT===== | ||
Combine 250μL DNA/ThT solution with 250μL 4% TEA 100mM DNA<br.> | Combine 250μL DNA/ThT solution with 250μL 4% TEA 100mM DNA<br.> | ||
Allow solution to react for 10 minutes<br.> | Allow solution to react for 10 minutes<br.> | ||
Follow same extraction procedure from | Follow same extraction procedure from [[http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/03/19| March 19, 2013]] | ||
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April 23, 2013Absorbance and Florescence Data of thiol DNA and ThT
Preparation of Gel and SamplesProcedure from Allison Alix on [2013/03/29] Attached AuNP to thiol DNA with ThTCombine 250μL DNA/ThT solution with 250μL 4% TEA 100mM DNA<br.> Allow solution to react for 10 minutes<br.> Follow same extraction procedure from [March 19, 2013]
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