User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/04/16: Difference between revisions
From OpenWetWare
| Line 8: | Line 8: | ||
==April 16, 2013== | ==April 16, 2013== | ||
=====Reconstituting DNA and Supernatants===== | =====Reconstituting DNA and Supernatants===== | ||
10μL of water used to reconstitute each sample of DNA and each of the four supernatants | |||
=====Gel Electrophoresis===== | |||
Procedure from Allison Alix 2013/04/16 [[http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/04/16]] | |||
=====Calculations for thiol DNA and ThT solution===== | |||
*AuNP starting concentration: 19.4nM | |||
*DNA starting concentration: 53.1μM | |||
DNA required starting concentration: 5.82μM | |||
(53.1μM)V=(5.82μM)(500μL) | |||
V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer | |||
*thiol DNA concentration required when combined with AuNP: 75×(19.4nM)=1455nM | |||
Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT | |||
*DNA concentration: 2.91μM | |||
*ThT concentration: 2.5μM | |||
Procedure continued on April 23, 2013 | |||
Revision as of 07:55, 24 April 2013
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
April 16, 2013Reconstituting DNA and Supernatants10μL of water used to reconstitute each sample of DNA and each of the four supernatants Gel ElectrophoresisProcedure from Allison Alix 2013/04/16 [[1]] Calculations for thiol DNA and ThT solution
DNA required starting concentration: 5.82μM (53.1μM)V=(5.82μM)(500μL) V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer
Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT
Procedure continued on April 23, 2013
| |
