User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/04/16: Difference between revisions
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*AuNP starting concentration: 19.4nM | *AuNP starting concentration: 19.4nM | ||
*DNA starting concentration: 53.1μM | *DNA starting concentration: 53.1μM | ||
DNA required starting concentration: 5.82μM | DNA required starting concentration: 5.82μM <br.> | ||
(53.1μM)V=(5.82μM)(500μL) | (53.1μM)V=(5.82μM)(500μL)<br.> | ||
V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer | V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer<br.> | ||
*thiol DNA concentration required when combined with AuNP: 75×(19.4nM)=1455nM | *thiol DNA concentration required when combined with AuNP: 75×(19.4nM)=1455nM<br.> | ||
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Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT | Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT | ||
*DNA concentration: 2.91μM | *DNA concentration: 2.91μM | ||
*ThT concentration: 2.5μM | *ThT concentration: 2.5μM | ||
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Procedure continued on April 23, 2013 | Procedure continued on April 23, 2013<br.> | ||
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Revision as of 07:56, 24 April 2013
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April 16, 2013Reconstituting DNA and Supernatants10μL of water used to reconstitute each sample of DNA and each of the four supernatants Gel ElectrophoresisProcedure from Allison Alix 2013/04/16 [[1]] Calculations for thiol DNA and ThT solution
DNA required starting concentration: 5.82μM <br.> (53.1μM)V=(5.82μM)(500μL)<br.> V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer<br.>
<br.> Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT
<br.> Procedure continued on April 23, 2013<br.> <br.> |