User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/26

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(March 26, 2013)
Current revision (11:36, 2 April 2013) (view source)
(serial dilutions done with 50mM HEPES buffer:)
 
Line 11: Line 11:
(9.7nM)V=(4.85nM)(100μL)<br.>
(9.7nM)V=(4.85nM)(100μL)<br.>
V= 50μL 9.7nM AuNP in 50μL<br.>
V= 50μL 9.7nM AuNP in 50μL<br.>
-
=====serial dilutions done with 50mM HEPES buffer:=====
+
=====Serial Dilutions with 50mM HEPES buffer:=====
4.85nM<br.>
4.85nM<br.>
2.425nM<br.>
2.425nM<br.>
Line 17: Line 17:
0.61nM<br.>
0.61nM<br.>
0.303nM<br.>
0.303nM<br.>
 +
=====FCS Data=====
=====FCS Data=====
*0.3nM
*0.3nM

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March 26, 2013

Dilutions of AuNP, MB, and thiol DNA Solution

Goal: dilute solution to 1-0.5nM AuNP
(9.7nM)V=(4.85nM)(100μL)
V= 50μL 9.7nM AuNP in 50μL

Serial Dilutions with 50mM HEPES buffer:

4.85nM
2.425nM
1.2125nM
0.61nM
0.303nM

FCS Data
  • 0.3nM

Τ=0.00018 ms
N= 0.41

  • 0.61nM

Τ=184.456 ms
N= 620

  • 0.3nM (trial 2)

Τ=0.2046 ms
N= 12

  • o.61nM (trial 2)

Τ=0
N=0

Prepare Smaller Intervals of Diluted Samples:

(2.425nM)V=(0.5nM)(50μL)
V=10.3μL in 39.7μL HEPES buffer
0.4 nM → 8.2μL in 41.75μL
0.3 nM → 6.2μL in 43.8μL
0.2 nM → 4.1μL in 45.95μL




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