User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/26

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(Prepare Smaller Intervals of Diluted Samples:)
(March 26, 2013)
Line 8: Line 8:
==March 26, 2013==
==March 26, 2013==
=====Dilutions of AuNP, MB, and thiol DNA Solution=====
=====Dilutions of AuNP, MB, and thiol DNA Solution=====
-
Goal: dilute solution to 1-0.5nM AuNP
+
Goal: dilute solution to 1-0.5nM AuNP<br.>
-
(9.7nM)V=(4.85nM)(100μL)
+
(9.7nM)V=(4.85nM)(100μL)<br.>
-
V= 50μL 9.7nM AuNP in 50μL
+
V= 50μL 9.7nM AuNP in 50μL<br.>
=====serial dilutions done with 50mM HEPES buffer:=====
=====serial dilutions done with 50mM HEPES buffer:=====
-
4.85nM
+
4.85nM<br.>
-
2.425nM
+
2.425nM<br.>
-
1.2125nM
+
1.2125nM<br.>
-
0.61nM
+
0.61nM<br.>
-
0.303nM
+
0.303nM<br.>
=====FCS Data=====
=====FCS Data=====
*0.3nM
*0.3nM
-
Τ=0.00018 ms
+
Τ=0.00018 ms<br.>
N= 0.41
N= 0.41
*0.61nM
*0.61nM
-
Τ=184.456 ms
+
Τ=184.456 ms<br.>
N= 620
N= 620
*0.3nM (trial 2)
*0.3nM (trial 2)
-
Τ=0.2046 ms
+
Τ=0.2046 ms<br.>
N= 12
N= 12
*o.61nM (trial 2)
*o.61nM (trial 2)
-
Τ=0
+
Τ=0<br.>
N=0
N=0
=====Prepare Smaller Intervals of Diluted Samples:=====
=====Prepare Smaller Intervals of Diluted Samples:=====
-
(2.425nM)V=(0.5nM)(50μL)
+
(2.425nM)V=(0.5nM)(50μL)<br.>
-
V=10.3μL in 39.7μL HEPES buffer
+
V=10.3μL in 39.7μL HEPES buffer<br.>
-
0.4 nM → 8.2μL in 41.75μL
+
0.4 nM → 8.2μL in 41.75μL<br.>
-
0.3 nM → 6.2μL in 43.8μL
+
0.3 nM → 6.2μL in 43.8μL<br.>
-
0.2 nM → 4.1μL in 45.95μL
+
0.2 nM → 4.1μL in 45.95μL<br.>

Revision as of 11:35, 2 April 2013

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March 26, 2013

Dilutions of AuNP, MB, and thiol DNA Solution

Goal: dilute solution to 1-0.5nM AuNP
(9.7nM)V=(4.85nM)(100μL)
V= 50μL 9.7nM AuNP in 50μL

serial dilutions done with 50mM HEPES buffer:

4.85nM
2.425nM
1.2125nM
0.61nM
0.303nM

FCS Data
  • 0.3nM

Τ=0.00018 ms
N= 0.41

  • 0.61nM

Τ=184.456 ms
N= 620

  • 0.3nM (trial 2)

Τ=0.2046 ms
N= 12

  • o.61nM (trial 2)

Τ=0
N=0

Prepare Smaller Intervals of Diluted Samples:

(2.425nM)V=(0.5nM)(50μL)
V=10.3μL in 39.7μL HEPES buffer
0.4 nM → 8.2μL in 41.75μL
0.3 nM → 6.2μL in 43.8μL
0.2 nM → 4.1μL in 45.95μL




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