User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/26: Difference between revisions
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(9.7nM)V=(4.85nM)(100μL) | (9.7nM)V=(4.85nM)(100μL) | ||
V= 50μL 9.7nM AuNP in 50μL | V= 50μL 9.7nM AuNP in 50μL | ||
=====serial dilutions done with 50mM HEPES buffer:===== | |||
4.85nM | 4.85nM | ||
2.425nM | 2.425nM | ||
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0.61nM | 0.61nM | ||
0.303nM | 0.303nM | ||
=====FCS Data===== | |||
*0.3nM | *0.3nM | ||
Τ=0.00018 ms | Τ=0.00018 ms | ||
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Τ=0 | Τ=0 | ||
N=0 | N=0 | ||
=====Prepare Smaller Intervals of Diluted Samples:===== | |||
(2.425nM)V=(0.5nM)(50μL) | (2.425nM)V=(0.5nM)(50μL) | ||
V=10.3μL in 39.7μL HEPES buffer | V=10.3μL in 39.7μL HEPES buffer |
Revision as of 09:34, 2 April 2013
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March 26, 2013Dilutions of AuNP, MB, and thiol DNA SolutionGoal: dilute solution to 1-0.5nM AuNP (9.7nM)V=(4.85nM)(100μL) V= 50μL 9.7nM AuNP in 50μL serial dilutions done with 50mM HEPES buffer:4.85nM 2.425nM 1.2125nM 0.61nM 0.303nM FCS Data
Τ=0.00018 ms N= 0.41
Τ=184.456 ms N= 620
Τ=0.2046 ms N= 12
Τ=0 N=0 Prepare Smaller Intervals of Diluted Samples:(2.425nM)V=(0.5nM)(50μL) V=10.3μL in 39.7μL HEPES buffer 0.4 nM → 8.2μL in 41.75μL 0.3 nM → 6.2μL in 43.8μL 0.2 nM → 4.1μL in 45.95μL
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