User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/26

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(March 26, 2013)
Line 11: Line 11:
(9.7nM)V=(4.85nM)(100μL)
(9.7nM)V=(4.85nM)(100μL)
V= 50μL 9.7nM AuNP in 50μL
V= 50μL 9.7nM AuNP in 50μL
-
======serial dilutions done with 50mM HEPES buffer:======
+
=====serial dilutions done with 50mM HEPES buffer:=====
4.85nM
4.85nM
2.425nM
2.425nM
Line 17: Line 17:
0.61nM
0.61nM
0.303nM
0.303nM
-
======FCS Data======
+
=====FCS Data=====
*0.3nM
*0.3nM
Τ=0.00018 ms
Τ=0.00018 ms
Line 30: Line 30:
Τ=0
Τ=0
N=0
N=0
-
======Prepare Smaller Intervals of Diluted Samples:======
+
=====Prepare Smaller Intervals of Diluted Samples:=====
(2.425nM)V=(0.5nM)(50μL)
(2.425nM)V=(0.5nM)(50μL)
         V=10.3μL in 39.7μL HEPES buffer
         V=10.3μL in 39.7μL HEPES buffer

Revision as of 12:34, 2 April 2013

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March 26, 2013

Dilutions of AuNP, MB, and thiol DNA Solution

Goal: dilute solution to 1-0.5nM AuNP (9.7nM)V=(4.85nM)(100μL) V= 50μL 9.7nM AuNP in 50μL

serial dilutions done with 50mM HEPES buffer:

4.85nM 2.425nM 1.2125nM 0.61nM 0.303nM

FCS Data
  • 0.3nM

Τ=0.00018 ms N= 0.41

  • 0.61nM

Τ=184.456 ms N= 620

  • 0.3nM (trial 2)

Τ=0.2046 ms N= 12

  • o.61nM (trial 2)

Τ=0 N=0

Prepare Smaller Intervals of Diluted Samples:

(2.425nM)V=(0.5nM)(50μL)

        V=10.3μL in 39.7μL HEPES buffer

0.4 nM → 8.2μL in 41.75μL 0.3 nM → 6.2μL in 43.8μL 0.2 nM → 4.1μL in 45.95μL




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