User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/26: Difference between revisions
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==March 26, 2013== | ==March 26, 2013== | ||
=====Dilutions of AuNP, MB, and thiol DNA Solution===== | =====Dilutions of AuNP, MB, and thiol DNA Solution===== | ||
Goal: dilute solution to 1-0.5nM AuNP | Goal: dilute solution to 1-0.5nM AuNP<br.> | ||
(9.7nM)V=(4.85nM)(100μL) | (9.7nM)V=(4.85nM)(100μL)<br.> | ||
V= 50μL 9.7nM AuNP in 50μL | V= 50μL 9.7nM AuNP in 50μL<br.> | ||
===== | =====Serial Dilutions with 50mM HEPES buffer:===== | ||
4.85nM | 4.85nM<br.> | ||
2.425nM | 2.425nM<br.> | ||
1.2125nM | 1.2125nM<br.> | ||
0.61nM | 0.61nM<br.> | ||
0.303nM | 0.303nM<br.> | ||
=====FCS Data===== | =====FCS Data===== | ||
*0.3nM | *0.3nM | ||
Τ=0.00018 ms | Τ=0.00018 ms<br.> | ||
N= 0.41 | N= 0.41 | ||
*0.61nM | *0.61nM | ||
Τ=184.456 ms | Τ=184.456 ms<br.> | ||
N= 620 | N= 620 | ||
*0.3nM (trial 2) | *0.3nM (trial 2) | ||
Τ=0.2046 ms | Τ=0.2046 ms<br.> | ||
N= 12 | N= 12 | ||
*o.61nM (trial 2) | *o.61nM (trial 2) | ||
Τ=0 | Τ=0<br.> | ||
N=0 | N=0 | ||
=====Prepare Smaller Intervals of Diluted Samples:===== | =====Prepare Smaller Intervals of Diluted Samples:===== | ||
(2.425nM)V=(0.5nM)(50μL) | (2.425nM)V=(0.5nM)(50μL)<br.> | ||
V=10.3μL in 39.7μL HEPES buffer | V=10.3μL in 39.7μL HEPES buffer<br.> | ||
0.4 nM → 8.2μL in 41.75μL | 0.4 nM → 8.2μL in 41.75μL<br.> | ||
0.3 nM → 6.2μL in 43.8μL | 0.3 nM → 6.2μL in 43.8μL<br.> | ||
0.2 nM → 4.1μL in 45.95μL | 0.2 nM → 4.1μL in 45.95μL<br.> | ||
Revision as of 09:36, 2 April 2013
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March 26, 2013Dilutions of AuNP, MB, and thiol DNA SolutionGoal: dilute solution to 1-0.5nM AuNP<br.> (9.7nM)V=(4.85nM)(100μL)<br.> V= 50μL 9.7nM AuNP in 50μL<br.> Serial Dilutions with 50mM HEPES buffer:4.85nM<br.> 2.425nM<br.> 1.2125nM<br.> 0.61nM<br.> 0.303nM<br.> FCS Data
Τ=0.00018 ms<br.> N= 0.41
Τ=184.456 ms<br.> N= 620
Τ=0.2046 ms<br.> N= 12
Τ=0<br.> N=0 Prepare Smaller Intervals of Diluted Samples:(2.425nM)V=(0.5nM)(50μL)<br.> V=10.3μL in 39.7μL HEPES buffer<br.> 0.4 nM → 8.2μL in 41.75μL<br.> 0.3 nM → 6.2μL in 43.8μL<br.> 0.2 nM → 4.1μL in 45.95μL<br.>
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