User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/26: Difference between revisions
From OpenWetWare
(Autocreate 2013/03/26 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence) |
|||
(4 intermediate revisions by the same user not shown) | |||
Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==March 26, 2013== | ||
* | =====Dilutions of AuNP, MB, and thiol DNA Solution===== | ||
Goal: dilute solution to 1-0.5nM AuNP<br.> | |||
(9.7nM)V=(4.85nM)(100μL)<br.> | |||
V= 50μL 9.7nM AuNP in 50μL<br.> | |||
=====Serial Dilutions with 50mM HEPES buffer:===== | |||
4.85nM<br.> | |||
2.425nM<br.> | |||
1.2125nM<br.> | |||
0.61nM<br.> | |||
0.303nM<br.> | |||
=====FCS Data===== | |||
*0.3nM | |||
Τ=0.00018 ms<br.> | |||
N= 0.41 | |||
*0.61nM | |||
Τ=184.456 ms<br.> | |||
N= 620 | |||
*0.3nM (trial 2) | |||
Τ=0.2046 ms<br.> | |||
N= 12 | |||
*o.61nM (trial 2) | |||
Τ=0<br.> | |||
N=0 | |||
=====Prepare Smaller Intervals of Diluted Samples:===== | |||
(2.425nM)V=(0.5nM)(50μL)<br.> | |||
V=10.3μL in 39.7μL HEPES buffer<br.> | |||
0.4 nM → 8.2μL in 41.75μL<br.> | |||
0.3 nM → 6.2μL in 43.8μL<br.> | |||
0.2 nM → 4.1μL in 45.95μL<br.> | |||
Revision as of 09:36, 2 April 2013
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
March 26, 2013Dilutions of AuNP, MB, and thiol DNA SolutionGoal: dilute solution to 1-0.5nM AuNP<br.> (9.7nM)V=(4.85nM)(100μL)<br.> V= 50μL 9.7nM AuNP in 50μL<br.> Serial Dilutions with 50mM HEPES buffer:4.85nM<br.> 2.425nM<br.> 1.2125nM<br.> 0.61nM<br.> 0.303nM<br.> FCS Data
Τ=0.00018 ms<br.> N= 0.41
Τ=184.456 ms<br.> N= 620
Τ=0.2046 ms<br.> N= 12
Τ=0<br.> N=0 Prepare Smaller Intervals of Diluted Samples:(2.425nM)V=(0.5nM)(50μL)<br.> V=10.3μL in 39.7μL HEPES buffer<br.> 0.4 nM → 8.2μL in 41.75μL<br.> 0.3 nM → 6.2μL in 43.8μL<br.> 0.2 nM → 4.1μL in 45.95μL<br.>
|